Antitumour agents must kill off cancer cells while protecting healthy tissue and having no toxic side-effects.

A novel approach based on “self-immolative” polyferrocenes - copolymers that split apart into their components as soon as they enter a tumour cell - could meet these demands.

The drugs they hold then synergistically cause an abrupt increase in free radicals and incapacitate the defenses of tumour cells, as reported by a research team in the journal Angewandte Chemie.

A team led by Xianglong Hu and Shiyong Liu at the University of Science and Technology of China (Hefei, China) incorporated two synergistically cooperative molecules together into a copolymer.

The copolymer chains are hydrophobic at one end and hydrophilic at the other. In aqueous environments, they aggregate into nanoparticles in which the hydrophobic ends are all pointed toward the inside and the outer, hydrophilic portions, are shielded by polyethylene glycol moieties.

Polyethylene glycol is a nontoxic polymer that is widely used in cosmetics and pharmaceuticals.

The polyethylene glycol layer prevents the nanoparticles from being rapidly broken up in the blood by components of the immune system.

In healthy tissues, the nanoparticles remain intact and inactive.

Only the significantly more acidic environment of tumour cells triggers the process of “self-immolation”.

The particles fall apart and the hydrophobic ends of the polymer chains split apart into their individual building blocks. These are made from azaquinone methide (AQM) units carrying aminoferrocene sidechains. Aminoferrocene is a special iron complex, a so-called sandwich complex: the iron atom is the “filling” between two flat, aromatic, five-membered carbon rings as the “slices of bread”.

Within the tumour cells, glutathione is subsequently activated.

Glutathione is a free radical trap and antioxidant that helps to remove foreign substances from cells.

Glutathione attacks the azaquinone methide units, binds to them, and splits off the iron sandwiches.

Once released, this complex reacts with hydrogen peroxide (H2O2) present in the cells to form hydroxyl radicals (•OH).

In this process the divalent iron in the complex is oxidized to trivalent iron, which the glutathione reduces back to FeII - a fatal cycle that consumes the glutathione and abruptly produces a high concentration of hydroxyl radicals in the cell.

The synergy of these two processes puts the tumour cells under severe oxidative stress, which damages and kills them.

Experiments performed by the team both in vitro and in mice with tumours demonstrated that this efficiently inhibited tumour growth with negligible side-effects.

This approach could allow for new possibilities in the chemodynamic therapy (CDT) of tumours.

Source: Wiley